Part 7: Immune System Series | Oral Tolerance to Foods
Summary
Active immune responses directed against the microbiota can result in inflammatory bowel diseases such as Crohn’s disease and ulcerative colitis. The ability of orally administered antigens to suppress subsequent immune responses, both in the gut and in the systemic immune system, is referred to as “oral tolerance.”
Every day the intestine is exposed to foreign antigenic material from the consumption of food. The intestine is also colonized with a dense community of commensal microbes, which are also foreign, referred to as the microbiota. All of this foreign material finds itself in the gastrointestinal tract where only a thin single cell layer of epithelial cells is the barrier between self and non-self. Thus, the intestinal immune system has to discriminate between generating protective immunity against harmful antigens and tolerance against harmless materials.1-2
Active immune responses directed against the microbiota can result in inflammatory bowel diseases such as Crohn’s disease and ulcerative colitis. Failure to induce tolerance to food protein is thought to result in food allergy and celiac disease, which is the most prevalent food-induced pathology.4
The ability of orally administered antigens to suppress subsequent immune responses, both in the gut and in the systemic immune system, is referred to as “oral tolerance.” Tolerance to food protein induced via the small intestine affects local and systemic immune responses; tolerance to gut bacteria in the colon does not reduce systemic responses.2 Oral tolerance is often used interchangeably to describe tolerance to food protein and bacteria; however, experts believe that this arbitrary terminology is potentially misleading.
While the gut microbiota and their metabolites impact host immunity, tolerance to orally administered soluble antigens influences mucosally induced tolerance that can be induced by other antigens in different parts of the intestine.4-5
Gut-associated lymphoid tissue (GALT) found in the small intestine epithelium includes Peyer’s patches, isolated lymphoid follicles, and microfold cells (M-cells), which mediate transcellular transport of particulate material including intestinal microbiota.2,6
Antigens that pass through the intestinal villous M-cells are passed on to lymphocytes called antigen-presenting dendritic cells (DCs) that reside just below the M-cells or in a “pocket” created at the basolateral surface of the M-cell. Upon exposure to the particulate matter, surrounding epithelial cells can attract more DCs via their production of CCL20, which can attract more DCs via their expression of a chemokine receptor (CCR6). Expression of CCR6, activates pathogen-specific T cells in Peyer’s patches.7
Pathogenic bacteria and dead cells are internalized by a unique subset of Peyer’s patch dendritic cells that express lysozyme.8 Specialized retinoic acid-dependent DCs (CD103+) in the lamina propria underlying the normal villus epithelium also plays a critical role in presenting antigen for the induction of tolerance.1 The CD103+ DC’s may acquire soluble antigens that diffuse through the tight junctions between epithelial villus cells or that have transferred across epithelial cells by passing through the villus by transcellular mechanisms.
Cell-derived vesicles called exosomes containing antigen derived from class II MHC+ enterocytes may be taken up by DCs. In addition, specialized macrophages (CX3CR1) have also been reported to capture luminal antigens by extending processes through the epithelial layer and then passing them on to neighboring CD103+ DCs. The antigen presenting cells are very important in stimulating a balanced immune response such as removal of harmful pathogens versus induction of tolerance to beneficial bacteria and to non-pathogenic environmental and dietary antigens.
Read part 8 of the Immune System Series: Strategies to Support Gut Health.
- Weiner, H. L., Cunha, A.P., et al. (2005). Oral tolerance. Immunological reviews 206: 232-259.
- Pabst, O., Mowat, A.M., (2012). Oral tolerance to food protein. Mucosal immunology 5(3): 232-239.
- Stamnaes, J., Sollid, L.M. (2015). Celiac disease: autoimmunity in response to food antigen. Seminars in Immunology 27(5): 343-352.
- Rooks, M. G., Garrett, M.S. (2016). Gut microbiota, metabolites and host immunity. Nat Rev Immunol 16(6): 341-352.
- Kim, D., Zeng, M.Y., et al. (2017). The interplay between host immune cells and gut microbiota in chronic inflammatory diseases. Exp Mol Med 49: e339.
- Fagarasan, S., Honjo, T. (2003). Intestinal IgA synthesis: regulation of front-line body defences. Nature reviews. Immunology 3(1): 63-72.
- Salazar-Gonzalez, R. M., Niess, J.H., et al. (2006). CCR6-mediated dendritic cell activation of pathogen-specific T cells in Peyer’s patches. Immunity 24(5): 623-632.
- Lelouard, H., Henri, S., et al. (2010). Pathogenic bacteria and dead cells are internalized by a unique subset of Peyer’s patch dendritic cells that express lysozyme. Gastroenterology 138(1): 173-184 e171-173.
